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Effect of different media on optimum growth and ability to produce alcohol of yeast strains isolated from domestic rainbow trout


مطلب مرتبط :   مدرسه، تبریز، دانشآموزان،

Unicellular yeast belongs to the Kingdom of Fungi which is naturally found in most environments. They also are known as microflora of animal’s intestine. These unicellular are verey impotant as bitechonologic material in medicin and food industry which has been used as natural probiotics. Due to these impacts they are on the center of attention of many years. Isolation, culture contion and also study on the applications of these valuable unicellular are the some main research topics on the world. Owing to the understating of culture condion of endemic unicellular yeasts and also fermentation rate in some strain and species of yeast this research topic was defined. There for several fish samples were obtained from 6 different rain bow trout fish farms around Urmia city. Isolation and charachterization of the yeast samples was provided by culture specific and common media as SDA, SCC, CHA, CMA, YPD, YPAD, YPG, YPAC and DM. Using theis study several strain and species of S.cerevisiae strain LN, S.cerevisiae strain YG3-1, Saccharomyces cerevisi, Kazachstania sp., IMBBR1-3, Kazachstania sp. IMBBR1-2, Kazachstania sp. IMBBR1-1, C. guilliermondii were identified as micro flora of these fishes. In the present study also the optimum growth condition condtion of all populations were seen in YPAD media with exception to C. guilliermondii which is found in YPAD and YPD media. In this research work ethanol production was studied in YPAD Media. The alcohole determination was provided by gas chromatography method (GC) in the media. In the present study the highest etanol production was found in Kazachstania sp. IMBBR1-3 and Kazachstania sp. strains (p<0.05). The present study over all could indentified several new strain and species of yeast as intestine microflora of Oncorhynchus mykiss fishes which with optimum growth condition of each. These results could be used then for culture of the found yeast samples after understating of probiotic or biotechnologic properties of these samples.

مطلب مرتبط :   اصول حاکم بر اسناد الکترونیکی و انواع سند الکترونیکی
Keywords: Intestine flora, Isolation, Rainbow trout, Media, alcohol Yeast.